Ascorbic Acid: A Potent Agent for Mitochondrial Damage Repair in H2O2 Treated Bone Marrow Mesenchymal Stromal Cells : Ascorbic Acid: Agent for Mitochondrial Damage Repair

Rabia  Mahmood; Sana Javaid  Awan; Lahraseb  Khan; Sabeen  Malik; Nida  Naeem; Amna  Mahmood; Laraib  Qamar

doi:10.54393/tt.v4i02.85

Authors

Rabia Mahmood Institute of Molecular Biology and Biotechnology, University of Lahore, Pakistan
Sana Javaid Awan Institute of Molecular Biology and Biotechnology, University of Lahore, Pakistan
Lahraseb Khan Institute of Molecular Biology and Biotechnology, University of Lahore, Pakistan
Sabeen Malik Institute of Molecular Biology and Biotechnology, University of Lahore, Pakistan
Nida Naeem Faculty of Science and Technology, University of Central Punjab, Lahore, Pakistan
Amna Mahmood Faculty of Science and Technology, University of Central Punjab, Lahore, Pakistan
Laraib Qamar Institute of Dentistry, CMH Lahore Medical College, Lahore, Pakistan

DOI:

https://doi.org/10.54393/tt.v4i02.85

Keywords:

Muscle Marrow Mesenchymal Stromal Cells, Ascorbic Acid, Mitochondrial Damage

Abstract

Bone Marrow Mesenchymal stromal cells (BMSCs) have shown an encouraging promise for cell-based treatments and regenerative medicine applications. Reactive oxygen species (ROS) can damage mitochondria and are detrimental to BMSC cell viability. Ascorbic acid, or vitamin C, is a crucial ingredient that is frequently added to culture media as an antioxidant. Its role in the proliferation of BMSCs has already been studied. However, no research has been done on its effects on the ability of BMSC to regenerate mitochondrial damage. Objective: To analyze the recovery of mitochondrial damage by H₂O₂-induced oxidative stress with Ascorbic Acid. Methods: BMSCs were cultured and treated with H₂O₂in order to induce oxidative stress. The injured BMSCs were then treated with vitamin C and their regeneration and recovery from mitochondrial damage is investigated by cell viability assays, ELISA and gene expression profiling. Recovery from oxidative damage is checked through anti-oxidative enzymes. Results: Findings showed that supplementing with vitamin C greatly enhanced cell viability and proliferation. It significantly decreased the BMSC's generation of ROS brought on by H₂O₂. These results imply that Ascorbic Acid may enhance the rate of proliferation and reduces apoptosis by recovering the mitochondrial damage as evidenced by the down-regulation of BAX. Conclusions: H₂O₂ when given to BMSC could create oxidative stress which in turn damages these cells as evidenced by their decreased cell viability. Ascorbic acid was also observed to regenerate the cells from H₂O₂ injury with the help of increased cells’ viability and proliferation and decreased apoptosis.

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